Journal: Human Reproduction Open

Article Title: Aging promotes accumulation of senescent and multiciliated cells in human endometrial epithelium

doi: 10.1093/hropen/hoae048

Figure Lengend Snippet: Schematic overview of the study design and workflow of the analysis. Endometrial biopsy samples were collected from 22 young maternal age (YMA) and 22 advanced maternal age (AMA) women on Day 5 of progesterone administration (P + 5, artificial cycle) undergoing endometrial receptivity testing using 68 transcriptomic markers and the beREADY algorithm. The study samples underwent mRNA sequencing (12 YMA and 12 AMA samples) and small RNA sequencing (11 YMA and 11 AMA samples), and validation samples (10 YMA and 10 AMA) were used for qPCR validation. Additionally, 24 women donated endometrial samples in the natural cycle. For the analyses, FFPE tissue blocks were prepared for all study samples and haematoxylin and eosin-stained slides were evaluated by the pathologist. Immunohistochemistry (IHC) analysis for p16 INK4a , C2CD4B, AcTubA, and LhS28 proteins was performed in endometrial samples of both age groups. Sequencing data processing included quality control, adaptor trimming, and alignment to the human genome. Aligned reads were counted and analysed for differential expression of genes between the YMA and AMA groups. Computational deconvolution analysis was performed using single-cell mRNA sequencing data from six endometrial cell populations. The figure was created with BioRender.com.

Article Snippet: To evaluate the expression of cellular senescence protein p16 INK4a (p16, CDKN2A), immunohistochemistry (IHC) analysis was performed, using anti-p16 INK4a , mouse monoclonal antibody against human CDKN2A (ready-to-use solution, Master Diagnostica, Valencia, Spain).

Techniques: Sequencing, RNA Sequencing, Biomarker Discovery, Staining, Immunohistochemistry, Control, Quantitative Proteomics

Journal: Human Reproduction Open

Article Title: Aging promotes accumulation of senescent and multiciliated cells in human endometrial epithelium

doi: 10.1093/hropen/hoae048

Figure Lengend Snippet: Genes dysregulated in the endometrium of women of advanced maternal age. ( A ) The top differentially expressed (DE) genes and their biological functions, FC > 1, Benjamin–Hochberg adjusted P -value < 0.05. ( B ) Molecular interaction network generated using Ingenuity pathway analysis (IPA). One of the top significant networks (score 35) suggests the interaction between the p16 ( CDKN2A ) senescence marker with Notch, ERK and Hedgehog signalling. ( C ) Functional analysis of DE genes in advanced maternal age (AMA) samples represent gene ontology (GO) terms significantly enriched in the endometrium of AMA samples compared to young maternal age (YMA) samples. The most significantly enriched terms were cilium (GO: 0005929, P = 2.42×10 −20 ), cilium movement (GO: 0003341, P = 4.74×10 −17 ), axoneme (GO: 0005930, P = 2.00×10 −17 ), and motile cilium (GO: 0031514, P = 1.44×10 −17 ). BP, biological processes; CC, cellular component; HP, human phenotype; MF, molecular function; REAC, reactome; TF, transcription factor; HPA, Human Protein Atlas; WP, WikiPathways. GO terms marked with numbers are described in . ( D and E ) Immunohistochemistry (IHC) analysis of p16 (p16 INK4a ) senescence protein in YMA and AMA samples, respectively. ( F ) Negative control staining for p16, in which the primary antibody was omitted. ( G ) p16 INK4a protein expression in endometrial cellular compartments, identified by IHC from YMA patients and AMA patients. p16 exhibited significantly higher expression in luminal epithelial cells (LE) and glandular epithelial cells (GE) (Wilcoxon rank-sum test P < 0.05).

Article Snippet: To evaluate the expression of cellular senescence protein p16 INK4a (p16, CDKN2A), immunohistochemistry (IHC) analysis was performed, using anti-p16 INK4a , mouse monoclonal antibody against human CDKN2A (ready-to-use solution, Master Diagnostica, Valencia, Spain).

Techniques: Generated, Marker, Functional Assay, Immunohistochemistry, Negative Control, Staining, Expressing

Journal: Romanian Journal of Morphology and Embryology

Article Title: Uterine myxoid leiomyosarcoma – a rare malignant tumor: the role of complex morphopathological assay. Review and case presentation

doi: 10.47162/RJME.62.4.01

Figure Lengend Snippet: Immunohistochemical panel of antibodies

Article Snippet: Anti-p16 , Invitrogen , 1D7D2 , Citrate, pH 6 , Monoclonal mouse anti-p16 INK4a , 1:50 , Human protein CDKN2A – present in HPV infection.

Techniques: Immunohistochemical staining, Infection, Marker

Journal: Romanian Journal of Morphology and Embryology

Article Title: Uterine myxoid leiomyosarcoma – a rare malignant tumor: the role of complex morphopathological assay. Review and case presentation

doi: 10.47162/RJME.62.4.01

Figure Lengend Snippet: Microscopic aspects of the tumor: (A) Cells that have undergone mutations in the p53 tumor suppressor gene, immunolabeled in brown; (B) Heavily reactive tumor cells are observed at p16, suggesting a high degree of inactivation of the tumor suppressor gene CDKN2A and an increase in the degree of tumor aggressiveness; (C and D) The density of cells in cell division, stained in brown, is observed. Immunomarking with anti-p53 antibody: (A) ×100. Immunomarking with anti-p16 antibody: (B) ×100. Immunomarking with anti-Ki67 antibody: (C) ×200; (D) ×400. CDKN2A: Cyclin-dependent kinase inhibitor 2A

Article Snippet: Anti-p16 , Invitrogen , 1D7D2 , Citrate, pH 6 , Monoclonal mouse anti-p16 INK4a , 1:50 , Human protein CDKN2A – present in HPV infection.

Techniques: Immunolabeling, Staining

Journal: The Scientific World Journal

Article Title: HPV Infection in Esophageal Squamous Cell Carcinoma and Its Relationship to the Prognosis of Patients in Northern China

doi: 10.1155/2014/804738

Figure Lengend Snippet: (a) In situ hybridization signal of HPV-positive esophageal squamous cell carcinomas. Numerous tumor cells show positive nuclear signals. (b) Immunohistochemical staining of p16 INK4A in esophageal squamous cell carcinomas. More than 50% of tumor cells showing strong nuclear and cytoplasm immunolabeling. (Original magnification ×200.)

Article Snippet: Processions were carried out by the Dako Envision-System method (code: GK500705) using a primary antibody against p16 (monoclonal mouse anti-human p16 INK4A protein, Clone G175-405, Dako).

Techniques: In Situ Hybridization, Immunohistochemical staining, Staining, Immunolabeling

Journal: The Scientific World Journal

Article Title: HPV Infection in Esophageal Squamous Cell Carcinoma and Its Relationship to the Prognosis of Patients in Northern China

doi: 10.1155/2014/804738

Figure Lengend Snippet: Correlation between HPV in situ hybridization and p16 immunohistochemistry in esophageal squamous cell carcinoma.

Article Snippet: Processions were carried out by the Dako Envision-System method (code: GK500705) using a primary antibody against p16 (monoclonal mouse anti-human p16 INK4A protein, Clone G175-405, Dako).

Techniques: In Situ Hybridization, Immunohistochemistry

Journal: The Scientific World Journal

Article Title: HPV Infection in Esophageal Squamous Cell Carcinoma and Its Relationship to the Prognosis of Patients in Northern China

doi: 10.1155/2014/804738

Figure Lengend Snippet: Kaplan-Meier estimates of survival among the study patients, according to tumor HPV status or p16-expression status. For 5-year overall survival rate (a) and 5-year progression-free survival rate (b), HPV was significantly associated with improved outcomes ( P = 0.002, P = 0.001, resp.). For 5-year OS rate (c) and 5-year progression-free survival rate (d), p16 was significantly associated with improved outcomes ( P = 0.021, P = 0.007, resp.).

Article Snippet: Processions were carried out by the Dako Envision-System method (code: GK500705) using a primary antibody against p16 (monoclonal mouse anti-human p16 INK4A protein, Clone G175-405, Dako).

Techniques: Expressing